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德国莱布尼茨神经生物研究所 联合成像中心主管 Leibniz Institute for Neurobiology, Germany. Head of the Joint Imaging Center
研究员 Research Fellow
贾宏博研究员专注于开发和应用脑科学中的物理学新技术方法,擅长双光子显微成像技术,曾参与策划我国多项国家级、省部级重大科学仪器研制计划和科技基础设施建设,并支持国际上多个脑科学研究机构的先进显微成像技术的部署应用。
Professor Hongbo Jia focuses on the development and application of new technologies and methods of physics in brain science, specializes in two-photon microscopy imaging technology, has participated in the planning of a number of national, provincial and ministerial major scientific instrument development plans and scientific and technological infrastructure construction in China, and supported the deployment and application of advanced microscopy imaging technology in many international brain science research institutions.
报告题目:
Finding long-term memory storage cells in the neocortex by using light and electricity
用光和电寻找新皮层中的长期记忆存储细胞
报告摘要:
本次演讲我们将探讨神经科学中一个古老但尚未解决的基本问题:感觉新皮质中到底哪些确切的细胞扮演了长期记忆存储的角色?
感觉新皮层被认为是长期记忆储存的基础,但究竟是哪些细胞储存记忆仍然不清楚,也没有可靠的追踪。特别是,最常用的印痕标记方法(由诺贝尔奖得主 利根川进 首创)使用即时早期基因(IEGs)无法解释地在感觉新皮层中不起作用。我们通过结合 双光子Ca2+成像 和 靶向单细胞电生理学,采取了不同的体内细胞生理学方法。我们最近发现,听觉皮层2/3层的稀疏细胞从静止状态进入一种独特的高频爆发模式,对习得的整体复合声音做出反应,而不是对单个组成音调做出反应。因此,这些细胞被称为“Holistic Bursting”细胞(HB细胞)。进一步的研究表明,HB细胞是习得的与行为相关的复合声音的印迹,因为相同的HB细胞满足Richard Wolfgang Semon在一个世纪前创造的印迹的所有四个定义标准。回顾过去,我们在感觉新皮层中发现的静止新生HB细胞作为长期记忆的候选物,也解释了为什么活动依赖的IEG标记不能做到这一点的未解之谜。
This talk addresses an age-old yet unsolved fundamental question in neuroscience: which exact cells act as long-term memory storage in the sensory neocortex?
The sensory neocortex has been proposed to be a substrate for long-term memory storage, yet which exact cells store memory remained neither known nor robustly traceable. Particularly, the most commonly established method (pioneered by Susumu Tonegawa, Nobel Prize Laureate) of engram tagging using immediate early genes (IEGs) unexplainably does not work in the sensory neocortex. We have taken a different approach of in-vivo cellular physiology by combining two-photon Ca2+ imaging and targeted single-cell electrophysiology. We recently reported that sparse cells in layer 2/3 of in auditory cortex emerged from quiescence into a unique mode of high-frequency bursting responses to a learned composite sound as a whole but not to individual constituent tones. Hence, these cells were referred to as “Holistic Bursting” cells (HB cells). Further investigations reveal that HB cells are engrams of the learned behaviorally-relevant composite sounds, as the same HB cells satisfy all the 4 defining criteria of an engram coined by Richard Wolfgang Semon a century ago. Retrospectively, our finding of the quiescence-emerging HB cells as long-term memory storage candidates in the sensory neocortex also explains the unexplained mystery of why activity-dependent IEG tagging has not been capable to do so.
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贾宏博研究员专注于开发和应用脑科学中的物理学新技术方法,擅长双光子显微成像技术,曾参与策划我国多项国家级、省部级重大科学仪器研制计划和科技基础设施建设,并支持国际上多个脑科学研究机构的先进显微成像技术的部署应用。
Professor Hongbo Jia focuses on the development and application of new technologies and methods of physics in brain science, specializes in two-photon microscopy imaging technology, has participated in the planning of a number of national, provincial and ministerial major scientific instrument development plans and scientific and technological infrastructure construction in China, and supported the deployment and application of advanced microscopy imaging technology in many international brain science research institutions.
报告题目:
Finding long-term memory storage cells in the neocortex by using light and electricity
用光和电寻找新皮层中的长期记忆存储细胞
报告摘要:
本次演讲我们将探讨神经科学中一个古老但尚未解决的基本问题:感觉新皮质中到底哪些确切的细胞扮演了长期记忆存储的角色?
感觉新皮层被认为是长期记忆储存的基础,但究竟是哪些细胞储存记忆仍然不清楚,也没有可靠的追踪。特别是,最常用的印痕标记方法(由诺贝尔奖得主 利根川进 首创)使用即时早期基因(IEGs)无法解释地在感觉新皮层中不起作用。我们通过结合 双光子Ca2+成像 和 靶向单细胞电生理学,采取了不同的体内细胞生理学方法。我们最近发现,听觉皮层2/3层的稀疏细胞从静止状态进入一种独特的高频爆发模式,对习得的整体复合声音做出反应,而不是对单个组成音调做出反应。因此,这些细胞被称为“Holistic Bursting”细胞(HB细胞)。进一步的研究表明,HB细胞是习得的与行为相关的复合声音的印迹,因为相同的HB细胞满足Richard Wolfgang Semon在一个世纪前创造的印迹的所有四个定义标准。回顾过去,我们在感觉新皮层中发现的静止新生HB细胞作为长期记忆的候选物,也解释了为什么活动依赖的IEG标记不能做到这一点的未解之谜。
This talk addresses an age-old yet unsolved fundamental question in neuroscience: which exact cells act as long-term memory storage in the sensory neocortex?
The sensory neocortex has been proposed to be a substrate for long-term memory storage, yet which exact cells store memory remained neither known nor robustly traceable. Particularly, the most commonly established method (pioneered by Susumu Tonegawa, Nobel Prize Laureate) of engram tagging using immediate early genes (IEGs) unexplainably does not work in the sensory neocortex. We have taken a different approach of in-vivo cellular physiology by combining two-photon Ca2+ imaging and targeted single-cell electrophysiology. We recently reported that sparse cells in layer 2/3 of in auditory cortex emerged from quiescence into a unique mode of high-frequency bursting responses to a learned composite sound as a whole but not to individual constituent tones. Hence, these cells were referred to as “Holistic Bursting” cells (HB cells). Further investigations reveal that HB cells are engrams of the learned behaviorally-relevant composite sounds, as the same HB cells satisfy all the 4 defining criteria of an engram coined by Richard Wolfgang Semon a century ago. Retrospectively, our finding of the quiescence-emerging HB cells as long-term memory storage candidates in the sensory neocortex also explains the unexplained mystery of why activity-dependent IEG tagging has not been capable to do so.